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KMID : 0380020150300040197
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 4 p.197 ~ p.201
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Simultaneous Enantiomer Separation of α-Amino Acids and Their Esters as Fluorenylmethoxycarbonyl Derivatives under UV and Fluorescence Detection by High Performance Liquid Chromatography
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Islam Md. Fokhrul
Lee Won-Jae
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Abstract
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Liquid chromatographic enantiomer separation of α-amino acids and their methyl and ethyl esters as fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a recently developed chiral column (Chiralpak IE) based on polysaccharide derivative under simultaneous UV detection and fluorescence detection. The degree of enantiomer separation of α-amino acid esters as FMOC derivatives is generally higher than that of the corresponding α-amino acids. Especially, α-amino acid methyl esters showed the greatest enantioseparation. As this method developed in this study can be applied to determine the chemical and optical purity of α- amino acids and esters, it is expected to be quite useful for their chiral separation using Chiralpak IE.
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KEYWORD
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Amino acid derivative, Chiral column, Enantiomer separation, Fluorenylmethoxycarbonyl derivative
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